Fig. 2From: Novel sporozoite-based ELISpot assay to assess frequency of parasite-specific B cells after vaccination with irradiated sporozoitesCSP-immune PBMC react with sporozoite lysates in an ELISpot format. Wells were either coated with sporozoite lysate (30,000/well, salivary gland preparation) or recombinant CSP protein (positive control) or BSA (negative control) and titrations of CSP-immune PBMC (indicated on x-axis) plated to determine the optimal cell concentration. Data (representative experiment) are expressed as the average number of reactive B cells (error represents SD of triplicate wells)Back to article page