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Fig. 2 | Malaria Journal

Fig. 2

From: Novel sporozoite-based ELISpot assay to assess frequency of parasite-specific B cells after vaccination with irradiated sporozoites

Fig. 2

CSP-immune PBMC react with sporozoite lysates in an ELISpot format. Wells were either coated with sporozoite lysate (30,000/well, salivary gland preparation) or recombinant CSP protein (positive control) or BSA (negative control) and titrations of CSP-immune PBMC (indicated on x-axis) plated to determine the optimal cell concentration. Data (representative experiment) are expressed as the average number of reactive B cells (error represents SD of triplicate wells)

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