Fig. 3From: Novel sporozoite-based ELISpot assay to assess frequency of parasite-specific B cells after vaccination with irradiated sporozoitesEnrichment of B cells prior to plating onto the sporozoite-ELISpot improves the assay sensitivity. Three CSP-immune donors (a, b, and c representing the donors) were tested to measure the parasite response for pre-immune, post 3 (i.e., pre challenge), and post challenge (4 weeks after controlled human malaria infection) time points. 5 × 105 PBMC/well (black symbol) or 105 purified B cells/well (white symbol) were plated. Data expressed as mean (SD) of triplicate wellsBack to article page