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Fig. 3 | Malaria Journal

Fig. 3

From: Primaquine–quinoxaline 1,4-di-N-oxide hybrids with action on the exo-erythrocytic forms of Plasmodium induce their effect by the production of reactive oxygen species

Fig. 3

Determination of total Glutathione levels in HepG2-CD81 cells infected with P. yoelii 17XNL or uninfected and treated with hybrid PQ–QdNO compounds. Representative chromatograms and retention times (ac) of Thiolyte and Thiolyte-GSH adducts. a Blank sample (cell-free), shows retention time (tr) of Thiolyte monobromobimane in 29.9 min of running. b Chromatogram of HepG2-CD81 uninfected control (Adduct Thiolyte-GSH tr: 30.4). c Chromatogram of HepG2-CD81 control infected with P. yoelii 17XNL (Adduct Thiolyte-GSH tr: 30.4). Line graphs (df) show the change in GSHt concentration in HepG2-CD81 cells infected with P. yoelii 17XNL or uninfected treated with PQ (d), and hybrids 6a (e) and 6b (f). The data are expressed as the average of three independent experiments. **p < 0.01 shows a statistically significant comparison between infected and uninfected groups. #p < 0.05 shows significant differences between control and treated in the uninfected group

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