Table 1 Primer sequence of Pfmdr1 gene flanking at codon 86 and 1246
From: Asymptomatic malaria infections and Pfmdr1 mutations in an endemic area of Nigeria
Primer | Sequence (5′ → 3′) | Direction | PCR product (bp) | Cycling condition |
|---|---|---|---|---|
Pfmdr1 86 and 184 | AGAGAAAAAAGATGGTAACCTCAG | Forward | 590 | 95 °C for 3 min, 34 cycles of 95 °C for 45 s, 60.2 °C for 45 s, and 72 °C for 5 min |
ACCACAAACATAAATTAACGG | Reverse | |||
Pfmdr1 1246 | GTGGAAAATCAACTTTTATGA | Forward | 499 | 94 °C for 5 min, 35 cycles of 94 °C for 1 min, 53 °C for 1 min, 72 °C for 1 min |
TTAGGTTCTCTTAATAATGCT | Reverse | |||
GACTTGAAAAATGATCACATT | Forward nested | 409 | 94 °C for 5 min, 35 cycles of 94 °C for 1 min, 50 °C for 1 min, 72 °C for 1 min | |
GTCCACCTGATAAGCTTTT | Reverse nested |