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Fig. 2 | Malaria Journal

Fig. 2

From: Improvement of an in vitro drug selection method for generating transgenic Plasmodium berghei parasites

Fig. 2

Generation of mutants using bsdPB driven by pbef- promoter. a Nucleotide sequences of original (bsd) and codon-optimized bsd (bsdPB). In the bsdPB sequence, modified nucleotides are highlighted. b Schematic diagram of the piggyBac transposon vector containing bsdPB and egfp expression cassettes (pXL/pbef-1α-bsdPB-egfp). ITR inverted terminal repeat. c The eGFP-positive parasite ratio after each blasticidin selection. The ratio was analysed using fluorescence microscopy. Each bar represents the mean ± SD of three independent experiments. **p < 0.01, n.s.: not significant (paired t-tests). d Fluorescence images of parasites after each selection. Parasites were stained with Hoechst 33342. The scale bar represents 10 µm. e Flow cytometry analysis of the typical transfection line of eGFP positive mutant ratio after each blasticidin selection. Numbers above the bracketed lines indicate the percentage of parasites with eGFP expression

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