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Fig. 3 | Malaria Journal

Fig. 3

From: Improvement of an in vitro drug selection method for generating transgenic Plasmodium berghei parasites

Fig. 3

Establishment of two cell-cycle culture selection method. a Schematic diagram of original in vitro selection method and two cell-cycle culture selection method. b The eGFP-positive parasite ratio after each puromycin selection performed by original method and two cell-cycle culture method. The ratio was analysed using fluorescence microscopy. Each bar represents the mean ± SD of four independent experiments. **p < 0.01, n.s.: not significant (paired t-tests). c Fluorescence images of parasites after each selection and two cell-cycle culture selection. Parasites were stained with Hoechst 33342. The scale bar represents 10 µm. d Flow cytometry analysis of the typical transfection line of eGFP positive mutant ratio after each puromycin selection and two cell-cycle culture method. Numbers above the bracketed lines indicate the percentage of parasites with eGFP expression

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