Fig. 3
From: Detection of low-density Plasmodium falciparum infections using amplicon deep sequencing
Identification of haplotypes in mock samples. a Detection of known haplotypes within the mock samples was dependent on the haplotype concentration (copies/μl) within the DNA sample. 5 μl of DNA template were used in the first round PCR amplification step prior to sequencing. Error bars represent the binomial-estimated standard deviation. b Across all mock samples, 31% of identified haplotypes were erroneous, but these haplotypes were generally supported by fewer reads than correct haplotypes. The number of nucleotide (nt) errors per haplotype was calculated as the nucleotide distance between an observed haplotype and the closest expected haplotype within the sample