Harmonization study between three laboratories for expression of malaria vaccine clinical trial IgG antibody ELISA data in µg/mL

Table 1 Statistical analysis of harmonized data

Laboratory	Oxford vs. WRAIR	Oxford vs. NIH	NIH vs. WRAIR
Concordance
Concordance coefficient (CC)	0.2987	0.8979	0.1693
Bootstrap 95% CI	(− 0.2105, 0.5635)	(0.5796, 0.9713)	(− 0.4382, 0.5359)
P value	0.2398	0.0064	0.6441
Linear regression
Slope	0.5034	0.9381	0.5095
Bootstrap 95% CI	(0.3663, 0.8545)	(0.7418, 1.3191)	(0.3490, 0.8517)
P value^a	0.0096	0.5933	0.0148
Correlation
Pearson’s correlation coefficient	0.9036	0.9489	0.904
Bootstrap 95% CI	(0.8098, 0.9820)	(0.8975, 0.9850)	(0.8023, 0.9927)
P value	0.0082	0.0002	0.0082

Bootstrap analysis of the new ELISA data in mass concentration scale was undertaken (n = 12) to assess for concordance. Linear regression and correlation analyses are also shown. The 95% confidence intervals (CI) were calculated using percentiles from 10,000 bootstraps
^aTesting the null hypothesis of slope = 1

ISSN: 1475-2875