Natural selection and genetic diversity of domain I of Plasmodium falciparum apical membrane antigen-1 on Bioko Island

Table 5 Function prediction scores of eight novel sites

Novel sites	Frequency (%)	Score (HumDiv)^a	Sensitivity	Specificity	Classification^b
P150T	2.29	1.000	0.00	1.00	Probably damaging
V151I	0.76	0.714	0.86	0.92	Probably damaging
I158S	1.53	0.998	0.27	0.99	Probably damaging
G180C	2.29	1.000	0.00	1.00	Probably damaging
A182V	0.76	1.000	0.00	1.00	Probably damaging
D266N	3.82	0.991	0.71	0.97	Probably damaging
S272N	0.76	0.846	0.83	0.93	Probably damaging
D281H	1.53	1.000	0.00	1.00	Probably damaging

^aHumDiv is preferred model for evaluating rare alleles, dense mapping of regions identified by genome-wide association studies, and analysis of natural selection
^bQualitative ternary classfication appraised at 5%/10% (HumDiv) FPR thresholds (“benign”, “possibly damaging”, “probably damaging”)

ISSN: 1475-2875