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Table 2 Results of image analysis, and estimation of antigen concentrations and type of malaria infection from Plasmodium vivax clinical samples

From: A two-colour multiplexed lateral flow immunoassay system to differentially detect human malaria species on a single test line

Sample no. 471 442 486 457 476
Microscopy examination Positive P. vivax Positive P. vivax Positive P. vivax Positive P. vivax Positive P. vivax
Red decay area at test line 631.10 846.20 826.30 907.39 857.67
Blue decay area at test line 299.22 416.34 356.00 445.82 409.38
Red/blue ratio of clinical samples (ratio_cal) 2.11 2.03 2.32 2.05 2.10
Estimated red/blue ratio at the red decay area (ratio_ref) 1.63 1.88 1.86 1.94 1.89
LFA colour discrimination Malaria positive, P. falciparum negative Malaria positive, P. falciparum negative Malaria positive, P. falciparum negative Malaria positive, P. falciparum negative Malaria positive, P. falciparum negative
LFA results (pLDH: μg mL−1) pLDH: 1.02 pLDH: 1.63 pLDH: 1.56 pLDH: 1.86 pLDH: 1.67
ELISA results (pLDH: μg mL−1; PfHRP2: ng mL−1) pLDH: 2.70
PfHRP2: negative
pLDH: *
PfHRP2: negative
pLDH: *
PfHRP2: 3.35
pLDH: *
PfHRP2: negative
pLDH: *
PfHRP2: negative
  1. * > 5.00 μg mL−1 but < 20.00 μg mL−1