A two-colour multiplexed lateral flow immunoassay system to differentially detect human malaria species on a single test line

Table 2 Results of image analysis, and estimation of antigen concentrations and type of malaria infection from Plasmodium vivax clinical samples

Sample no.	471	442	486	457	476
Microscopy examination	Positive P. vivax	Positive P. vivax	Positive P. vivax	Positive P. vivax	Positive P. vivax
Red decay area at test line	631.10	846.20	826.30	907.39	857.67
Blue decay area at test line	299.22	416.34	356.00	445.82	409.38
Red/blue ratio of clinical samples (ratio_cal)	2.11	2.03	2.32	2.05	2.10
Estimated red/blue ratio at the red decay area (ratio_ref)	1.63	1.88	1.86	1.94	1.89
LFA colour discrimination	Malaria positive, P. falciparum negative	Malaria positive, P. falciparum negative	Malaria positive, P. falciparum negative	Malaria positive, P. falciparum negative	Malaria positive, P. falciparum negative
LFA results (pLDH: μg mL⁻¹)	pLDH: 1.02	pLDH: 1.63	pLDH: 1.56	pLDH: 1.86	pLDH: 1.67
ELISA results (pLDH: μg mL⁻¹; PfHRP2: ng mL⁻¹)	pLDH: 2.70 PfHRP2: negative	pLDH: * PfHRP2: negative	pLDH: * PfHRP2: 3.35	pLDH: * PfHRP2: negative	pLDH: * PfHRP2: negative

ISSN: 1475-2875