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Fig. 1 | Malaria Journal

Fig. 1

From: Evaluation of Plasmodium falciparum MSP10 and its development as a serological tool for the Peruvian Amazon region

Fig. 1

The rMSP10 western blot. a P. falciparum Merozoite Surface Protein 10 (rMSP10) expressed in baculovirus: Lane 1: an Sf9 cell lysate from the recombinant baculovirus with the optimized pfmsp10 gene. Lane 2: The lysate of non-infected Sf9 cells. Lane 3: molecular weight protein marker (kDa). b rMSP10 purified: Eluted samples obtained from the purification process with imidazole: Lane 1: 50 mM; lane 2: 150 mM; lane 3: 200 mM; lane 4: 250 mM; lane 5: lysates from uninfected Sf9 cells; lane 6: lysates from Sf9 cells infected with the recombinant baculovirus with pfmsp10 gene; lanes 7–9: washes. Black arrows indicate rMSP10 (~ 56 kDa)

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