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Fig. 2 | Malaria Journal

Fig. 2

From: Enhanced uptake, high selective and microtubule disrupting activity of carbohydrate fused pyrano-pyranones derived from natural coumarins attributes to its anti-malarial potential

Fig. 2

Uptake of carbohybrid 12 by P. falciparum induces cell death. a (i) Cellular uptake images of parasitized erythrocytes at different time points from 30 min to 2 h of incubation with carbohybrid 12 (6 µM) and precursor compound 5 (6 µM) (ii) carbohybrid 12 treated parasitized cells for 2 h were incubated with 2-NBDG for 30 min. Carbohybrid 12 shows increase in uptake in the time dependent manner. Uninfected erythrocytes treated with carbohybrid 12 showed less uptake of compound as compared to parasitized erythrocytes. b Fluorescence images of parasites showing JC-1 aggregates (red) in the mitochondria and monomeric JC-1 (green) in the cytoplasm. The first two rows of images shows untreated parasites with bright red signal at 590 nm indicating a functional mitochondrion; the last two rows show carbohybrid 12 treated dead parasites with bright green and very faint red signal. The bar graph showing the ratio of JC-1 (red)/JC-1 (green) in parasite population after treatment with carbohybrid 12 w.r.t control is shown in right panel. Scale bar = 5 µm

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