Skip to main content
Fig. 5 | Malaria Journal

Fig. 5

From: Enhanced uptake, high selective and microtubule disrupting activity of carbohydrate fused pyrano-pyranones derived from natural coumarins attributes to its anti-malarial potential

Fig. 5

Carbohybrid 12 alters P. falciparum microtubules dynamics. a Parasites at ring and early trophozoite stages were treated with compounds for 24 h, permeabilized, stained with an anti-α-tubulin antibody followed by Alexa Fluor 546 secondary antibody (red). Nuclear material was stained with DAPI (blue). Panel (i) shows untreated P. falciparum erythrocyte. Panel (ii) shows parasite treated with carbohybrid 12 at 6 µM. Panel (iii) shows parasite treated with 500 nM paclitaxel for 24 h. Panel (iv) represent images of parasite treated with colchicine at 10 µM after 24 h. Carbohybrid 12 treated parasites showed diffuse staining of tubulin similar to colchicine. Scale bar = 1 µm. b Tubulin protein in presence of carbohybrid 12 shows thermostability at different temperature conditions varying from 50 to 70 ºC

Back to article page