Fig. 3
From: Ultrasensitive loop mediated isothermal amplification (US-LAMP) to detect malaria for elimination
Validation of P. falciparum specific US-LAMP assay on diluted clinical specimens. Pf-LAMP was carried out on whole blood (a, b) and dried blood spot (c, d) extracted total nucleic acid. Experiments were performed in triplicate where error bars indicate standard error of mean (SEM). Here again, the threshold time (min = minutes) was determined by placing the threshold bar at 200 RFU in the CFX96 Real Time system. Each number/mL is indicative of parasite count/mL and R2 indicates goodness of fit to the straight line. a and c represents one of the three corresponding experiments