Table 3 Sensitivity and specificity of the US-LAMP assay on symptomatic and asymptomatic specimen compared to RT-PCR
From: Ultrasensitive loop mediated isothermal amplification (US-LAMP) to detect malaria for elimination
Sample type | Assay type | Sensitivity (%) | 95% confidence interval (CI) | Specificity (%) | 95% confidence interval (CI) | Positive predictive value (PPV) (%) | 95% confidence interval (CI) | Negative predictive value (NPV) (%) | 95% confidence interval (CI) |
|---|---|---|---|---|---|---|---|---|---|
Symptomatic Calgary (N = 113) | Pan-LAMP | 100 | 89.3–100 | 98.6 | 91.5–99.9 | 97.6 | 85.9–99.9 | 100 | 93.6–100 |
Pf-LAMP | 100 | 82.8–100 | 97.8 | 91.4–99.6 | 92.3 | 73.4–98.7 | 100 | 94.7–100 | |
Asymptomatic Bangladesh (N = 186) | Pan-LAMP | 100 | 39.6–100 | 100 | 97.4–100 | 100 | 39.6–100 | 100 | 97.4–100 |
Pf-LAMP | 100 | 39.6–100 | 100 | 97.4–100 | 100 | 39.6–100 | 100 | 97.4–100 | |
Asymptomatic Ethiopia (N = 308) | Pan-LAMP | 96.6 | 80.4–99.8 | 98.6 | 96.1–99.5 | 87.5 | 70.0–95.9 | 99.6 | 97.7–100 |
Pf-LAMP | 100 | 69.9–100 | 99.7 | 97.8–100 | 92.3 | 62.1–99.6 | 100 | 98.4–100 | |
Overall asymptomatic (N = 494) | Pan-LAMP | 97 | 82.5–99.8 | 99.1 | 97.6–99.7 | 88.9 | 73.0–96.4 | 99.7 | 98.6–100 |
Pf-LAMP | 100 | 75.9–100 | 99.8 | 98.6–100 | 94.1 | 69.2–99.7 | 100 | 99.0–100 |