Fig. 3
Long term incubation of CQGREEN treated parasites. Early trophozoite stage 3D7 parasites were incubated with 100 nM, 300 nM or 500 nM CQGREEN for 7 h in culture medium at 37 °C, 3% O2, 5% CO2, and then transferred onto a microscope chamber. Faint fluorescence accumulation of CQGREEN in the DV could be detected in few parasites (approx. 5%). Experiments were done in triplicate. a–c Images of parasites exposed to 100 nM CQGREEN for 7 h. d–f Images of parasites exposed to 300 nM CQGREEN for 7 h. g–i Images of parasites exposed to 500 nM CQGREEN for 7 h. Scale bar, 5 µm