Prevalence and distribution of G6PD deficiency: implication for the use of primaquine in malaria treatment in Ethiopia

Table 1 PCR primer sequences and amplicon size of G6PD gene

PCR pair	Primer name	Primer sequence (5′–3′)	Primer position	PCR product (bp)	Coding region (bp)	Exon coverage
1	6284F	CAAGGAGTGATTTGGGCAAT	6284
	6755R	AGAGCAAAACTCCGTCTCCA	6755	472	120	Exon 3
2	D-6F	AATCTCGGGGCTCTTCTGTCTG	16,237
	D-1170R	GCAACGGCAAGCCTTACATCTG	17,422	1185	365	Exon 4–6
3	D-1880F	CTTCGGGAGGGACCTGCAGAG	18,107
	D-2865R	GTGGTGACTTCTCCGGGGTTGA	19,114	1007	379	Exon 7–9
4	G6-5F	CCTGAGGGCTGCACATCT	19,356
	G6-5R	GTGTCTTGCTGATGCCACTG	20,096	740	423	Exon 10–11

ISSN: 1475-2875