|
PCR pair
|
Primer name
|
Primer sequence (5′–3′)
|
Primer position
|
PCR product (bp)
|
Coding region (bp)
|
Exon coverage
|
|---|
|
1
|
6284F
|
CAAGGAGTGATTTGGGCAAT
|
6284
| | | |
| |
6755R
|
AGAGCAAAACTCCGTCTCCA
|
6755
|
472
|
120
|
Exon 3
|
|
2
|
D-6F
|
AATCTCGGGGCTCTTCTGTCTG
|
16,237
| | | |
| |
D-1170R
|
GCAACGGCAAGCCTTACATCTG
|
17,422
|
1185
|
365
|
Exon 4–6
|
|
3
|
D-1880F
|
CTTCGGGAGGGACCTGCAGAG
|
18,107
| | | |
| |
D-2865R
|
GTGGTGACTTCTCCGGGGTTGA
|
19,114
|
1007
|
379
|
Exon 7–9
|
|
4
|
G6-5F
|
CCTGAGGGCTGCACATCT
|
19,356
| | | |
| |
G6-5R
|
GTGTCTTGCTGATGCCACTG
|
20,096
|
740
|
423
|
Exon 10–11
|
- Primer position can be referred to NCBI accession no. NG_009015.2
