Fig. 3

Longitudinal analysis of anti-rhoptry responses. The reactive index (RI) of specific IgG responses to PvRALP1-Ecto and PvRhopH2 antigens in the plasma of P. vivax subjects during follow-up was detected by ELISA. a Anti-PvRALP1-Ecto and anti-PvRhopH2 responses at all time points in the cohort study: acute infection and 3, 9, and 12 months after infection (n = 18). The horizontal line represents mean values ± SEM. b, c The kinetics of antibody responses against PvRALP1-Ecto and PvRhopH2 in 14 seropositive P. vivax-infected patients at different time points: acute infection and 3, 9, and 12 months after infection. Solid lines indicate individual subjects who have antibody responses to PvRALP1-Ecto and PvRhopH2 up to 12 months. Dotted lines indicate individual subjects who have antibody responses to PvRALP1-Ecto and PvRhopH2 less than 12 months. Dashed line indicates the cut-off value (RI ≥ 1)