Fig. 1
Construction of pFastBac1â„¢ vectors and recombinant baculoviruses (rBV) generation. Plasmodium berghei AMA-1 gene and influenza M1 gene were cloned into pFastBac1â„¢ with EcoRI/I and EcoRI/XhoI enzymes, respectively, resulting in P. berghei AMA-1 plasmid (a) and M1 plasmid (b). M marker, O native(non)-codon optimized gene, G codon-optimized gene, 1 M1 gene. Normal SF9 cells (c) and recombinant baculoviruses expressing influenza M1, AMA-1 non-codon optimized or codon-optimized gene on day 4 (d)