Fig. 2

Mean percent survival ± standard deviation of Plasmodium falciparum strains Cam3.I^R539T and Cam3.I^rev after exposure to DHA, OZ277, OZ439 and OZ609 at clinically relevant compound exposure parameters. a Parasites were exposed for 6 h to 5- or 25-fold the IC90/MIC of DHA or OZ277 (30 nM or 149 nM DHA; 15 nM or 74 nM OZ277). b Parasites were exposed for 20 h to 25-fold or 48 h to 5-fold IC90/MIC of OZ439 or OZ609 (168 nM or 34 nM OZ439; 96 nM or 19 nM OZ609). After drug removal, parasites were first incubated for 22 h with hypoxanthine-free medium, and then for 24 h with medium containing [³H]hypoxanthine. After this [³H]hypoxanthine incorporation period, the assays were terminated by freezing the plates at − 20 °C. At least three biological replicates were performed in each case