Table 1 Primer sequences
Gene | Primer/probe | Sequence |
|---|---|---|
Pf18S | 18S forward | 5′GTAATTGGAATGATAGGAATTTACAAGGT 3′ |
Pf18S | 18S reverse | 5′TCAACTACGAACGTTTTAACTGCAAC 3′ |
Pf18S | 18S probe | 6FAM-AACAATTGGAGGGCAAG–MGBNFQ |
Dhps | Outer forward | 5′ GATTCTTTTTCAGATGGAGG 3′ |
Dhps | Outer reverse | 5′ TTCCTCATGTAATTCATCTGA 3′ |
Dhps | Inner forward | 5′ AACCTAAACGTGCTGTTCAA 3′ |
Dhps | Inner reverse | 5′ AATTGTGTGATTTGTCCACAA 3′ |
Dhfr | Outer forward | 5′ TGATGGAACAAGTCTGC 3′ |
Dhfr | Outer reverse | 5′ ACTTTGTTTATTTCCATTCA 3′ |
Dhfr | Inner forward | 5′ TGTGCATGTTGTAAGGTTGA 3′ |
Dhfr | Inner reverse | 5′ GATACTCATTTTCATTTATTTCTGGA 3′ |
Dhps | 540 K | 5′ ATTAAACAACTCTTAACTACACAAGGAAATCCACATACAATGGATA 3′ |
Dhps | 540 E | 5′ CTTTCTTAATACATTACAACATACGAAATCCACATACAATGGATG 3′ |
Dhps | 581 A | 5′ CATAAATCTTCTCATTCTAACAAATTGATATTGGATTAGGATTTGC 3′ |
Dhps | 581 G | 5′ CAAACAAACATTCAAATATCAATCTTGATATTGGATTAGGATTTGG 3′ |
Dhfr | 164 I | 5′ CATAAATCTTCTCATTCTAACAAAGAAATTAAATTACTATAAATGTTTTATTA 3′ |
Dhfr | 164 L | 5′ CAAACAAACATTCAAATATCAATCGAAATTAAATTACTATAAATGTTTTATTT 3′ |