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Fig. 3 | Malaria Journal

Fig. 3

From: IL35 modulation altered survival, cytokine environment and histopathological consequences during malaria infection in mice

Fig. 3Fig. 3Fig. 3Fig. 3Fig. 3Fig. 3

a Immunohistochemical staining showing the localization of IL-35p35 in mouse brain. Each panel is a representative photomicrograph from either 5 control mice (A, C, E) or 5 P. berghei infected mice (B, D, F). In P. berghei infected mouse brain (B, D, F) positive cytoplasmic staining (black arrows) was evident in the neurons of the cerebral cortex. Scale bar: 50 µm at ×400 total magnification. There was no immunoreactivity observed in the neurons of the cerebral cortex from uninfected mice (arrow heads). The signal was developed using HRP-labelled secondary antibody and DAB reagent, nuclei were counterstained with Meyer’s haematoxylin. Images shown are a representative from three experiments. b Immunohistochemical staining showing the localization of IL-35p35 in mouse liver. Each panel is a representative photomicrograph from either 5 control mice (A, C, E) or 5 P. berghei infected mice (B, D, F). In P. berghei infected mouse liver (B, D, F) positive cytoplasmic staining (black arrows) was evident in stellate macrophages (Kupffer cells) and epithelial cells lining the portal tract. Scale bar: 50 µm at ×400 total magnification. Marginal (constitutive) immunoreactivity was observed in uninfected mouse liver (arrow heads). The signal was developed using DAB reagent, nuclei were counterstained with Meyer’s haematoxylin. Images shown are a representative from three experiments. c Immunohistochemical staining showing the localization of IL-35p35 in mouse spleen. Each panel is a representative photomicrograph from either 5 control mice (A, C, E) or 5 P. berghei infected mice (B, D, F). In P. berghei infected mouse spleen (B, D, F) positive cytoplasmic staining (black arrows) was evident in a subset of splenocytes in spleen from P. berghei infected mice. Representative images were acquired at ×400 total magnification. Scale bar = 50 µm. Marginal (constitutive) immunoreactivity was observed in uninfected mouse spleen. The signal was developed using HRP-labelled secondary antibody and DAB reagent, nuclei were counterstained with Meyer’s haematoxylin. WP white pulp, RP red pulp, MZ marginal zone. Images shown are a representative from three experiments. d Immunohistochemical staining showing the localization of IL-35p35 in mouse kidney. Each panel is a representative photomicrograph from either 5 control mice (A, C, E) or 5 P. berghei infected mice (B, D, F). In P. berghei infected mouse kidney (B, D, F) positive cytoplasmic staining (black arrows) was evident in the renal cortex. Diffused membrane staining (immunoreactivity) was specifically localized to the renal tubules and intercalated cells of the collecting ducts. Scale bar: 50 µm at ×400 total magnification. Marginal constitutive immunoreactivity was observed in uninfected mouse kidney (arrow heads). Images shown are a representative from three experiments. e Immunohistochemical staining showing the localization of IL-35p35 in mouse lung. Each panel is a representative photomicrograph from either 5 control mice (A, C, E) or 5 P. berghei infected mice (B, D, F). In P. berghei infected mouse lung (B, D, F) positive cytoplasmic staining (black arrows) was evident in the epithelial cells lining the bronchioles, alveolar epithelium and epithelioid histiocytes (macrophages). Scale bar: 50 µm at ×400 total magnification. There was no immunoreactivity observed in uninfected mouse lung (arrow heads). The signal was developed using HRP-labelled secondary antibody and DAB reagent, nuclei were counterstained with Meyer’s haematoxylin. Images shown are a representative from three experiments. f Immunohistochemical staining showing the localization of IL-35p35 in mouse heart. Each panel is a representative photomicrograph from either 5 control mice (A, C, E) or 5 P. berghei infected mice (B, D, F). Scale bar: 50 µm at ×400 total magnification. The signal was developed using HRP-labelled secondary antibody and DAB reagent, nuclei were counterstained with Meyer’s haematoxylin. Images shown are a representative from three experiments

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