Table 1 Sequences of murine primers used in quantitative RT-PCR experiments
Gene | Sense | Antisense | Product size (bp) |
|---|---|---|---|
GAPDH | 5′-ACA-CAT-TGG-GGG-TAG-GAA-CA | 5′-AAC-TTT-GGC-ATT-GTG-GAA-GG | 222 |
IL-1β | 5′-GAT-CCA-CAC-TCT-CCA-GCT-GCA | 5′-CAA-CCA-ACA-AGT-GAT-ATT-CTC-GAT-G | 151 |
TNF | 5′-CTC-CCT-TTG-CAG-AAC-TCA-GG | 5′-AGC-CCC-CAG-TCT-GTA-TCC-TT | 211 |
HO-1 | 5′-CCA-GAG-TGT-TCA-TTC-GAG-CA | 5′-CAC-GCA-TAT-ACC-CGC-TAC-CT | 174 |
IL-12 | 5′-TGG-TTT-GAT–GAT-GTC-CCT-GA | 5′-AGG-TCA-CAC-TGG-ACC-AAA-GG | 172 |
IFNg | 5′-TGA-GCT-CAT-TGA-ATG-CTT-GG | 5′-ACT-GGC-AAA-AGG-ATG-GTG-AC | 236 |
CD11b | 5′-AGA-TCG-TCT-TGG-GAG-ATG-CT | 5′-GAC-TCA-GTC-AGC-CCC-ATC-AT | 169 |
TLR2 | 5′-TGT-AAC-GCA-ACA-GCT-TCA-GG | 5′-TGC-TTT-CCT-GCT-GGA-GAT-TT | 196 |
ICAM-1 | 5′-AGC-TTG-CAC-GAC-CCT-TCT-AA | 5′-AGC-ACC-TCC-CCA-CCT-ACT-TT | 159 |
GranzB | 5′-GCT-TCA-CAT-TGA-CAT-TGC-GC | 5′-AGA-ACA-GGA-GAA-GAC-CCA-GC | 172 |
NFkB | 5′-ACC-GAA-GCA-GGA-GCT-ATC-AA | 5′-GCG-TAC-ACA-TTC-TGG-GGA-GT | 178 |
PGEs | 5′-CAG-CCT-ATT-GTT-CAG-CGA-CA | 5′-CCT-AGG-CTT-CAG-CCT-CAC-AC | 157 |
TGFb | 5′-GAC-TCT-CCA-CCT-GCA-AGA-CC | 5′-ACG-CGG-GTG-ACC-TCT-TTA G | 246 |
CD36 | 5′-GAG-CAA-CTG-GTG-GAT-GGT-TT | 5′-GCA-GAA-TCA-AGG-GAG-AGC-AC | 206 |
iNOS | 5′-ACA-AGG-CCT-CCA-ATC-TCT-GC | 5′-TCC-TGG-ACA-TTA-CGA-CCC-CT | 95 |
VEGF | 5′-GCT-GTA-ACG-ATG-AAG-CCC-TG | 5′-CGC-TCC-AGG-ATT-TAA-ACC-GG | 236 |
P. berghei | 5′-TCA-TTG-GGC-TCT-CAA-AGG-GT | 5′-CAA-TTG-GAG-GGC-AAG-TCT-GG | 209 |