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Table 2 Key points of methodology used for detection of deletions in pfhrp2 and/or pfhrp3 genes

From: Prevalence of Plasmodium falciparum field isolates with deletions in histidine-rich protein 2 and 3 genes in context with sub-Saharan Africa and India: a systematic review and meta-analysis

AuthorsWere samples positive to reference diagnosis method?Has used RDT fulfilled WHO requirements? Targeted malarial antigensPCR-amplified pfhrpExon 1Exon 2Across exons 1–2Flanking regionsWas DNA quality of PCR-negative for HRP2/3 verified?Laboratory strains used as control?Elimination of low parasitemia from analysis?
Koita et al. [26]Yes (microscopy)Yes (ParaSight F), PfHRP2pfhrp2NoYesNoNoYes (msp1)NoNo
Wurtz et al. [27]Yes (microscopy + real time PCR)Yes (Palutop+4®), PfHRP2, PvLDH and pan LDHpfhrp2 and 3NoYesNoNoYes (independent further amplification of PfHRP-negative samples)NoNo
Amoah et al. [28]Yes (microscopy)Not applicablepfhrp2 and 3NoYesNoNoYes (msp2 and glurp)3D7, Dd2, HB3No
Beshir et al. [29]Yes (microscopy + real time PCR)Yes (SD Bioline® Pf Ag), PfHRP2pfhrp2 and 3YesYesNoNoYes (pfhrp3 and Pf Beta tubulin)NoNot applicable
Gupta et al. [30]Yes (real time PCR)Not applicablepfhrp2 and 3NoYesNoYesYes (pfk13, pfmdr1, pfcrt)3D7, Dd2, HB3No
Kozycki et al. [31]Yes (microscopy + 18sRNA qPCR)Yes (SD Bioline® Pf/Pan Ag), PfFHRP2 and pan-pLDHpfhrp2 and 3NoYesNoYesYes (msp 1 and 2)NoYes (qPCR)
Menegon et al. [32]Yes (microscopy + 18sRNA qPCR)Yes (SD Bioline® Pf Ag), PfHRP2pfhrp2 and 3NoYesNoNoYes (pfk13)3D7Not applicable
Parr et al. [33]Yes (microscopy)Yes (first response® Malaria), PfHRP2 and pLDHpfhrp2NoYesNoNoYes (18sRNA)NoNot applicable
Ranadive et al. [34]Yes (microscopy + qPCR)Not specifiedpfhrp2 and 3NoYesYesYesNot specifiedNot specifiedYes
Berhane et al. [35]Yes (microscopy)Yes (care start pan LDH)pfhrp2 and 3NoNoYesYesYes (msp 1, 2 and glurp)NoNo
Nderu et al. [36]Yes (microscopy + 18sRNA qPCR)Yes (care start HRP2/pLDH)pfhrp2 and 3NoYesNoNoYes (msp1)3D7, Dd2, HB3Not applicable
Willie et al. [37]Yes (PCR)Yes (SD Bioline® Pf/Pv Ag), PfFHRP2 and pan-pLDHpfhrp2YesYesNoNoNot specified3D7, Dd2, HB3Not applicable
Funwei et al. [38]Yes (microscopy + PCR)Yes (SD Bioline® Pf/Pv Ag), PfFHRP2 and pan-pLDHpfhrp2 and 3NoYesNoNoYes (msp 1 and 2)3D7, Dd2, HB3Not applicable
Kobayashi et al. [39]Yes (microscopy + PCR)Yes (SD Bioline® Pf/Pv Ag), PfFHRP2 and pan-pLDHpfhrp2 and 3NoYesYesYesYes (msp 1, 2 and glurp)3D7, Dd2Not applicable
Mussa et al. [40]Yes (microscopy)ICT Test™ malaria Pf/Pvpfhrp2NoYesNoNoNot specifiedNot specifiedNot specified
Thomson et al. [41]Yes (microscopy + PCR)Yes (ICT diagnostics and care start)pfhrp2 and 3NoYesNoNomspNot specifiedYes
Kumar et al. [42]Yes (microscopy)Yes (para check and SD Bioline Pf), PfHRP2pfhrp2 and 3NoYesYesYesYes (msp 1, 2 and glurp)3D7, Dd2Not applicable
Bharti et al. [43]Yes (microscopy)Yes (SD Bioline® Pf/Pv Ag), pfhrp2 and pan-pLDHpfhrp2 and 3NoYesYesYesYes (msp 1, 2 and 18sRNA)3D7, Dd2, HB3No
Pati et al. [44]Yes (microscopy)Yes (SD Bioline® Pf Ag), PfHRP2pfhrp2 and 3NoYesNoYesYes (msp 1 and 2)Yes (but not specified)No
  1. DNA: deoxyribonucleic acid; GLURP: glutamate-rich protein; HRP: histidine-rich protein; LDH: lactate dehydrogenase; MSP: merozoite surface protein; Pf: Plasmodium falciparum; pfcrt: P. falciparum chloroquine resistance transporter; pfk13: P. falciparum Kelch 13 gene; pfmdr 1: P. falciparum multidrug resistance 1; Pv: Plasmodium vivax; RNA: ribonucleic acid; SD: standard Diagnostics; qPCR: quantitative polymerase chain reaction WHO: World Health Organization