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Table 2 Key points of methodology used for detection of deletions in pfhrp2 and/or pfhrp3 genes

From: Prevalence of Plasmodium falciparum field isolates with deletions in histidine-rich protein 2 and 3 genes in context with sub-Saharan Africa and India: a systematic review and meta-analysis

Authors Were samples positive to reference diagnosis method? Has used RDT fulfilled WHO requirements? Targeted malarial antigens PCR-amplified pfhrp Exon 1 Exon 2 Across exons 1–2 Flanking regions Was DNA quality of PCR-negative for HRP2/3 verified? Laboratory strains used as control? Elimination of low parasitemia from analysis?
Koita et al. [26] Yes (microscopy) Yes (ParaSight F), PfHRP2 pfhrp2 No Yes No No Yes (msp1) No No
Wurtz et al. [27] Yes (microscopy + real time PCR) Yes (Palutop+4®), PfHRP2, PvLDH and pan LDH pfhrp2 and 3 No Yes No No Yes (independent further amplification of PfHRP-negative samples) No No
Amoah et al. [28] Yes (microscopy) Not applicable pfhrp2 and 3 No Yes No No Yes (msp2 and glurp) 3D7, Dd2, HB3 No
Beshir et al. [29] Yes (microscopy + real time PCR) Yes (SD Bioline® Pf Ag), PfHRP2 pfhrp2 and 3 Yes Yes No No Yes (pfhrp3 and Pf Beta tubulin) No Not applicable
Gupta et al. [30] Yes (real time PCR) Not applicable pfhrp2 and 3 No Yes No Yes Yes (pfk13, pfmdr1, pfcrt) 3D7, Dd2, HB3 No
Kozycki et al. [31] Yes (microscopy + 18sRNA qPCR) Yes (SD Bioline® Pf/Pan Ag), PfFHRP2 and pan-pLDH pfhrp2 and 3 No Yes No Yes Yes (msp 1 and 2) No Yes (qPCR)
Menegon et al. [32] Yes (microscopy + 18sRNA qPCR) Yes (SD Bioline® Pf Ag), PfHRP2 pfhrp2 and 3 No Yes No No Yes (pfk13) 3D7 Not applicable
Parr et al. [33] Yes (microscopy) Yes (first response® Malaria), PfHRP2 and pLDH pfhrp2 No Yes No No Yes (18sRNA) No Not applicable
Ranadive et al. [34] Yes (microscopy + qPCR) Not specified pfhrp2 and 3 No Yes Yes Yes Not specified Not specified Yes
Berhane et al. [35] Yes (microscopy) Yes (care start pan LDH) pfhrp2 and 3 No No Yes Yes Yes (msp 1, 2 and glurp) No No
Nderu et al. [36] Yes (microscopy + 18sRNA qPCR) Yes (care start HRP2/pLDH) pfhrp2 and 3 No Yes No No Yes (msp1) 3D7, Dd2, HB3 Not applicable
Willie et al. [37] Yes (PCR) Yes (SD Bioline® Pf/Pv Ag), PfFHRP2 and pan-pLDH pfhrp2 Yes Yes No No Not specified 3D7, Dd2, HB3 Not applicable
Funwei et al. [38] Yes (microscopy + PCR) Yes (SD Bioline® Pf/Pv Ag), PfFHRP2 and pan-pLDH pfhrp2 and 3 No Yes No No Yes (msp 1 and 2) 3D7, Dd2, HB3 Not applicable
Kobayashi et al. [39] Yes (microscopy + PCR) Yes (SD Bioline® Pf/Pv Ag), PfFHRP2 and pan-pLDH pfhrp2 and 3 No Yes Yes Yes Yes (msp 1, 2 and glurp) 3D7, Dd2 Not applicable
Mussa et al. [40] Yes (microscopy) ICT Test™ malaria Pf/Pv pfhrp2 No Yes No No Not specified Not specified Not specified
Thomson et al. [41] Yes (microscopy + PCR) Yes (ICT diagnostics and care start) pfhrp2 and 3 No Yes No No msp Not specified Yes
Kumar et al. [42] Yes (microscopy) Yes (para check and SD Bioline Pf), PfHRP2 pfhrp2 and 3 No Yes Yes Yes Yes (msp 1, 2 and glurp) 3D7, Dd2 Not applicable
Bharti et al. [43] Yes (microscopy) Yes (SD Bioline® Pf/Pv Ag), pfhrp2 and pan-pLDH pfhrp2 and 3 No Yes Yes Yes Yes (msp 1, 2 and 18sRNA) 3D7, Dd2, HB3 No
Pati et al. [44] Yes (microscopy) Yes (SD Bioline® Pf Ag), PfHRP2 pfhrp2 and 3 No Yes No Yes Yes (msp 1 and 2) Yes (but not specified) No
  1. DNA: deoxyribonucleic acid; GLURP: glutamate-rich protein; HRP: histidine-rich protein; LDH: lactate dehydrogenase; MSP: merozoite surface protein; Pf: Plasmodium falciparum; pfcrt: P. falciparum chloroquine resistance transporter; pfk13: P. falciparum Kelch 13 gene; pfmdr 1: P. falciparum multidrug resistance 1; Pv: Plasmodium vivax; RNA: ribonucleic acid; SD: standard Diagnostics; qPCR: quantitative polymerase chain reaction WHO: World Health Organization