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Fig. 8 | Malaria Journal

Fig. 8

From: Identification, molecular characterization and expression of aminopeptidase N-1 (APN-1) from Anopheles stephensi in SF9 cell line as a candidate molecule for developing a vaccine that interrupt malaria transmission

Fig. 8

AsAPN-1 bioactivity assay. Bioactivity assay of AsAPN-1 was performed in the presence of the 1,10-phenanthroline as the specific inhibitor, l-leucine p-nitroanilide as specific substrate and Streptomyces griseus aminopeptidase (Sigma) as positive control and standard. Bioactivity assay revealed that the recombinant AsAPN-1 could degrade the specific substrate and 1,10-phenanthroline (10 μM) as the metalloproteases inhibitor is able to bind specifically to the active site of recombinant AsAPN-1and disrupt its enzymatic activity. Optical density was measured at 405 nm after 15, 30, 60, 90 and 120 min after the start of reaction

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Malaria Journal

ISSN: 1475-2875

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