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Table 1 List of the primers designed and used in this study

From: Identification, molecular characterization and expression of aminopeptidase N-1 (APN-1) from Anopheles stephensi in SF9 cell line as a candidate molecule for developing a vaccine that interrupt malaria transmission

 

No.

Primer name

Sequence

5ʹ-Genome walking primers

1

GWA

5ʹ-GATCAGGCGTCGCGTACCTCNNCTACTG-3ʹʹ

2

GWB

5ʹ-GATCAGGCGTCGCGTACCTCNNCTACT-3ʹ

3

GWC

5ʹ-GATCAGGCGTCGCGTACCTCNNCTAC-3ʹ

4

GWD

5ʹ-GATCAGGCGTCGCGTACCTCNNCACGCA-3ʹ

5

GWE

5ʹ-GATCAGGCGTCGCGTACCTCNNCACGC-3ʹ

6

GWF

5ʹ-GATCAGGCGTCGCGTACCTCNNCACG-3ʹ

7

GWG

5ʹ-GATCAGGCGTCGCGTACCTCNNGAGAC-3ʹ

8

UAP-N1

5ʹ-CTGTGAGCAGTCGTATCCACCGATCAGGCGTCGCGTACCTC-3ʹ

9

UAP-N2

5ʹ-CCTGTGAGCAGTCGTATCCAC-3ʹ

10

GW 27-R

5ʹ-ACGGTGAACGTTGCCTTCAG-3ʹ

11

GW 40 –R

5ʹ-GTTTTCCCAGTCACGAC-3ʹ

12

GW 59-R

5ʹ-CGATATGGCACTGTAGGTAATGCTG-3ʹ

13

GW 130-R

5ʹ-GGCGTCTTGTCGAACACCG-3ʹ

14

GW 282-R

5ʹ-CTCGTCCAGCACCTTCAG-3ʹ

3ʹ-RACE primers

15

RC 921

5ʹ-CAGATCATGCGCACCTGGACCAACG-3ʹ

16

RC 1011

5ʹ-CTGTTGGCGTTCGTTGTGTC-3ʹ

17

RC 1200

5ʹ-CGAATTCTATGCCGCCCATCT-3ʹ

18

RC 1422

5ʹ-TCCTACTTCAACAGCCGTCTCC-3ʹ

19

RC 2196

5ʹ-CACAAGTACCTCGTGCAGAC-3ʹ

20

RC 2241

5ʹ-TGGGCTACAAGGACTGTCTG-3ʹ

21

RC 2328

5ʹ-CCACCGTCACGTACTGTTAC-3ʹ

22

RC 3146

5ʹ-CATCCCGAACACCACAACCG-3ʹ

23

Linker Oligo(dT)

5ʹ-GAGATTTGAATCTTGCTTCTGGGCCCTCTAT TGTCATTGTCTTTTTTTTTTTTTTTTT-3ʹ

24

Inner RC

5ʹ-GGCCCTCTATTGTCATTGTC-3ʹ

25

Outer RC

5ʹ-GAGATTTGAATCTTGCTTCTG-3ʹ

 

26

T7 RC

5ʹ-TAATACGACTCACTATAG-3ʹ

Full-length primers

27

FL inner-F

5ʹ-CGATTAGTGCACCACCGAGTCTGC-3ʹ

28

FL Outer-F

5ʹ-ATGGTGCGTTCGAAGATCCTAGCAG-3ʹ

29

FL inner-R

5ʹ-CCTAATCATCTCGATTCCGGAGCTCCTG-3ʹ

30

FL outer-R

5ʹ-TTATCCCAGTAGATGGACCGCG-3ʹ

Full-length cloning primers

33

UFLC-F

5ʹ-AAGCTTctATGGTGCGTTCGAAGATC-3ʹ

34

DFLC-R

5ʹ-CTCGAGtTTATCCCAGTAGATGGACCG-3ʹ