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Table 1 List of the primers designed and used in this study

From: Identification, molecular characterization and expression of aminopeptidase N-1 (APN-1) from Anopheles stephensi in SF9 cell line as a candidate molecule for developing a vaccine that interrupt malaria transmission

 No.Primer nameSequence
5ʹ-Genome walking primers1GWA5ʹ-GATCAGGCGTCGCGTACCTCNNCTACTG-3ʹʹ
2GWB5ʹ-GATCAGGCGTCGCGTACCTCNNCTACT-3ʹ
3GWC5ʹ-GATCAGGCGTCGCGTACCTCNNCTAC-3ʹ
4GWD5ʹ-GATCAGGCGTCGCGTACCTCNNCACGCA-3ʹ
5GWE5ʹ-GATCAGGCGTCGCGTACCTCNNCACGC-3ʹ
6GWF5ʹ-GATCAGGCGTCGCGTACCTCNNCACG-3ʹ
7GWG5ʹ-GATCAGGCGTCGCGTACCTCNNGAGAC-3ʹ
8UAP-N15ʹ-CTGTGAGCAGTCGTATCCACCGATCAGGCGTCGCGTACCTC-3ʹ
9UAP-N25ʹ-CCTGTGAGCAGTCGTATCCAC-3ʹ
10GW 27-R5ʹ-ACGGTGAACGTTGCCTTCAG-3ʹ
11GW 40 –R5ʹ-GTTTTCCCAGTCACGAC-3ʹ
12GW 59-R5ʹ-CGATATGGCACTGTAGGTAATGCTG-3ʹ
13GW 130-R5ʹ-GGCGTCTTGTCGAACACCG-3ʹ
14GW 282-R5ʹ-CTCGTCCAGCACCTTCAG-3ʹ
3ʹ-RACE primers15RC 9215ʹ-CAGATCATGCGCACCTGGACCAACG-3ʹ
16RC 10115ʹ-CTGTTGGCGTTCGTTGTGTC-3ʹ
17RC 12005ʹ-CGAATTCTATGCCGCCCATCT-3ʹ
18RC 14225ʹ-TCCTACTTCAACAGCCGTCTCC-3ʹ
19RC 21965ʹ-CACAAGTACCTCGTGCAGAC-3ʹ
20RC 22415ʹ-TGGGCTACAAGGACTGTCTG-3ʹ
21RC 23285ʹ-CCACCGTCACGTACTGTTAC-3ʹ
22RC 31465ʹ-CATCCCGAACACCACAACCG-3ʹ
23Linker Oligo(dT)5ʹ-GAGATTTGAATCTTGCTTCTGGGCCCTCTAT TGTCATTGTCTTTTTTTTTTTTTTTTT-3ʹ
24Inner RC5ʹ-GGCCCTCTATTGTCATTGTC-3ʹ
25Outer RC5ʹ-GAGATTTGAATCTTGCTTCTG-3ʹ
 26T7 RC5ʹ-TAATACGACTCACTATAG-3ʹ
Full-length primers27FL inner-F5ʹ-CGATTAGTGCACCACCGAGTCTGC-3ʹ
28FL Outer-F5ʹ-ATGGTGCGTTCGAAGATCCTAGCAG-3ʹ
29FL inner-R5ʹ-CCTAATCATCTCGATTCCGGAGCTCCTG-3ʹ
30FL outer-R5ʹ-TTATCCCAGTAGATGGACCGCG-3ʹ
Full-length cloning primers33UFLC-F5ʹ-AAGCTTctATGGTGCGTTCGAAGATC-3ʹ
34DFLC-R5ʹ-CTCGAGtTTATCCCAGTAGATGGACCG-3ʹ