Identification, molecular characterization and expression of aminopeptidase N-1 (APN-1) from Anopheles stephensi in SF9 cell line as a candidate molecule for developing a vaccine that interrupt malaria transmission

Table 7 Prediction of interactions of the AsAPN-1 and AgAPN-1 epitopes with MHC-II using pyDockWEB

An.species	Ranking	MHC DR B binding residues	ΔG	MHC DQA binding residues	ΔG	MHC DQB binding residues	ΔG
Stephensi	1	E579, D474, A478	− 34.713	T827, G834, Q293	− 30.475	R278, Y277, L276	− 38.209
	2	G529, Q424, V428	− 32.335	Y77, T784, N244	− 28.591	L229, S228, V227	− 36.053
	3	A629, R574, A528	− 32.028	L829, L884, T344	− 28.579	A328, I327, Q326	− 35.164
	4	W679, R574, A528	− 31.593	W933, V934, E394	− 27.632	F378, W377, Q376	− 34.910
Gambiae	1	E574, D469, A473	− 43.215	T821, I826, N288	− 42.264	R273, L272, Q271	− 40.466
	2	G524, Q419, G423	− 37.861	Y771, A778, K238	− 41.554	I223, S222, V221	− 33.475
	3	A623, K569, A523	− 34.736	L823, L878, T339	− 39.920	A323, I322, Q321	− 31.378
	4	W673, K569, A523	− 34.131	W937, V928, E389	− 39.837	F373, W372, Q371	− 28.125

ISSN: 1475-2875