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Fig. 3 | Malaria Journal

Fig. 3

From: A simple monochromatic flow cytometric assay for assessment of intraerythrocytic development of Plasmodium falciparum

Fig. 3

Intensity of VSG depends on the stage of intraerythrocytic development of P. falciparum.a Giemsa-stained thin blood smear of the non-synchronized culture revealed mixed developmental stages of falciparum malaria parasites. b Flow cytometric profile of P. falciparum infected erythrocytes. The non-synchronized culture of malaria parasites was stained with 10 µg/mL of VSG. Single cells were separated based on fluorescence intensity into high, intermediate, or low (hereafter referred to as VSGhigh, VSGintermediate, and VSGlow, respectively). c Morphology of Giemsa-stained VSGhigh, VSGintermediate, and VSGlow cells. d Higher magnification of P. falciparum-infected cells in the boxed area of VSGintermediate and VSGlow cells in Fig. 3c. e Cell granularity of VSGhigh, VSGintermediate, and VSGlow cells was assessed based on SSC-A. Scale bars: 10 µm for Fig. 3a, c, and 5 µm for Fig. 3d. FSC-A, forward scatter area; VSG, ViSafe Green

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