Table 1 Sequences of primers for msp1, msp2 and glurp genes of P. falciparum from Cameroon

Msp-1 (block 2)

 Primary PCR

CHM1-OF: 5′ CTAGAAGCTTTAGAAGATGCAGTATTG-3′

CHM1-OR: 5′ CTTAAATAGTATTCTAATTCAAGTGGATCA-3′

 Secondary PCR

CHM1-KF: 5′ AAATGAAGAAGAAATTACTACAAAAGGTGC-3′

CHM1-KR: 5′ GCTTGCATCAGCTGGAGGGCTTGCACCAGA-3′

CHM1-MF: 5′ AAATGAAGGAACAAGTGGAACAGCTGTTAC-3′

CHM1-MR: 5′ ATCTGAAGGATTTGTACGTCTTGAATTACC-3′

CHM1-RF: 5′TAAAGGATGGAGCAAATACTCAAGTTGTTG-3′

CHM1-RR: 5′ CATTTGAAGGATTTGCAGCACCTGGAGATC-3′

Msp-2 (block 3)

 Primary PCR

M2-OF: 5′ ATGAAGGTAATTAAAACATTGTCTATTATA-3′

M2-OR: 5′ CTTTGTTACCATCGGTACATTCTT’3′

 Secondary PCR

M2-FCF: 5′ ATATTAAGAGTGTAGGTGCARATGCTCCA-3′

M2-FCR: 5′ TTTTATTTGGTGCATTGCCAGAACTTGAAC-3′

M2-ICF: 5′AGAAGTATGGCAGAAAGTAAKCCTYCTACT-3′

M2-ICR: 5′ GATTGTAATTCGGGGGATTCAGTTTGTTCG-3′

Glurp (region II)

 Primary PCR

CHG-OF: 5′ -TGAATTTGAAGATGTTCACACTGAAC-3′ 3′

CHG-OR: 5′ -GTG GAATTGCTTTTTCTTCAACACTAA-3′

 Secondary PCR

CHG-NF: 5′-TGAATTTGA AGA TGT TCA CAC TGA AC-3′

CHG-OR: 5′-GTG GAATTGCTT TTTCTTCAACAC TAA-3′