Table 1 Sequence analysis between An. funestus multiplex PCR primers and the ITS2 region of member species of the An. gambiae complex
From: Member species of the Anopheles gambiae complex can be misidentified as Anopheles leesoni
An. funestus multiplex PCR primers | An. arabiensis ITS2* sequence | An. gambiae ITS2* sequence | An. merus ITS2* sequence | An. quadriannulatus* ITS2 sequence | ||||
|---|---|---|---|---|---|---|---|---|
% Identity with primer | Number of consecutive bases at 3ʹ end of primer that binds with sequence | % Identity with primer | Number of consecutive bases at 3ʹ end of primer that binds with sequence | % Identity with primer | Number of consecutive bases at 3ʹ end of primer that binds with sequence | % Identity with primer | Number of consecutive bases at 3ʹ end of primer that binds with sequence | |
UV | 100 | 19 | 100 | 19 | 100 | 19 | 100 | 19 |
FUN | 63 | 3 | 63 | 3 | 63 | 3 | 63 | 3 |
VAN | 57 | 3 | 57 | 3 | 57 | 3 | 57 | 3 |
RIV | 34 | 2 | 63 | 1 | 40 | 0 | 40 | 0 |
PAR | 64 | 4 | 64 | 4 | 64 | 4 | 64 | 4 |
RIVLIKE | 65 | 0 | 65 | 0 | 63 | 0 | 65 | 2 |
LEES | 77 | 7 | 53 | 7 | 53 | 7 | 53 | 7 |