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Table 1 Sequence analysis between An. funestus multiplex PCR primers and the ITS2 region of member species of the An. gambiae complex

From: Member species of the Anopheles gambiae complex can be misidentified as Anopheles leesoni

An. funestus multiplex PCR primersAn. arabiensis ITS2* sequenceAn. gambiae ITS2* sequenceAn. merus ITS2* sequenceAn. quadriannulatus* ITS2 sequence
% Identity with primerNumber of consecutive bases at 3ʹ end of primer that binds with sequence% Identity with primerNumber of consecutive bases at 3ʹ end of primer that binds with sequence% Identity with primerNumber of consecutive bases at 3ʹ end of primer that binds with sequence% Identity with primerNumber of consecutive bases at 3ʹ end of primer that binds with sequence
UV10019100191001910019
FUN633633633633
VAN573573573573
RIV342631400400
PAR644644644644
RIVLIKE650650630652
LEES777537537537
  1. *The ITS2 sequences of the An. gambiae complex species are: An. arabiensis ITS2 sequence (KT160245.1, GenBank); An. gambiae ITS2 sequence (KT120234.1, GenBank); An. merus ITS2 sequence (GQ870313.1, GenBank) and An. quadriannulatus ITS2 sequence (JN994146.1, GenBank)