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Table 2 Summary of the results from the different iterations of the Anopheles funestus multiplex PCR used to amplify DNA from members of the An. gambiae species complex

From: Member species of the Anopheles gambiae complex can be misidentified as Anopheles leesoni

Samples used for An. funestus multiplex PCR

~ 150 bp amplicon produced via An. funestus multiplex PCR

~ 150 bp amplicon produced by An. funestus multiplex PCR without LEES primer

~ 150 bp amplicon produced by An. funestus multiplex PCR with UV and LEES primers only

An. funestus

No—an amplicon of 500 bp was produced, which corresponds to the An. funestus amplicon

No—an amplicon of 500 bp was produced, which corresponds to the An. funestus amplicon

No

An. leesoni

Yes

No

Yes

No template negative control

No

No

No

Extraction kit negative control

No

No

No

An. arabiensis

Yes

No

Yes

An. gambiae

Yes

No

Yes

An. merus

Yes

No

Yes

An. quadriannulatus

Yes

No

Yes