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Table 2 Summary of the results from the different iterations of the Anopheles funestus multiplex PCR used to amplify DNA from members of the An. gambiae species complex

From: Member species of the Anopheles gambiae complex can be misidentified as Anopheles leesoni

Samples used for An. funestus multiplex PCR~ 150 bp amplicon produced via An. funestus multiplex PCR~ 150 bp amplicon produced by An. funestus multiplex PCR without LEES primer~ 150 bp amplicon produced by An. funestus multiplex PCR with UV and LEES primers only
An. funestusNo—an amplicon of 500 bp was produced, which corresponds to the An. funestus ampliconNo—an amplicon of 500 bp was produced, which corresponds to the An. funestus ampliconNo
An. leesoniYesNoYes
No template negative controlNoNoNo
Extraction kit negative controlNoNoNo
An. arabiensisYesNoYes
An. gambiaeYesNoYes
An. merusYesNoYes
An. quadriannulatusYesNoYes