Fig. 7

Metabolite and metabolic pathway concordance between this study and studies by Olszewski et al. [4] and Babbitt et al. [8]. a Temporal profiles of metabolite abundance quantified during the intraerythrocytic developmental cycle in the three studies. Metabolite abundances were normalized by their value at t = 0 h (grey vertical bar) to allow comparison across studies. Metabolites are grouped by metabolite class. b Spearman’s ρ computed for metabolites quantified in all three studies (N = 41) and within each metabolite class. The correlation for the lipid class, which contained only two metabolites, was not computed. c Spearman’s ρ computed for all metabolites at 8, 16, 24, 32, and 40 h. In comparisons with both studies, the correlation was near zero at the 8-h time point, i.e., when parasite metabolism is least active [73]. The dotted line shows the average (~ 0.3) of the correlations at each time point for both studies. ADP adenosine diphosphate, AMP adenosine monophosphate, CMP cytidine monophosphate, DHAP dihydroxyacetone phosphate, \( \widehat{\text{FC}} \) metabolite abundance normalized with respect to t = 0 h, GMP guanosine monophosphate, IMP inosine monophosphate, NAD⁺ nicotinamide adenine dinucleotide (oxidized), PEP phosphoenolpyruvate, UDP uridine diphosphate, UMP uridine monophosphate