Fig. 8

Distribution of significantly altered metabolites among major metabolic pathways at 0–8 h, 16–24 h, and 32–40 h. Significantly altered metabolites (p ≤ 0.05; q < 0.10) were identified by performing a two-way analysis of variance on the metabolomic data from the cell fractions of uninfected and infected cultures at the indicated time points. At the earliest time points (0–8 h) most of the significantly altered metabolites belonged to the lipid class, but at later time points (16–24 h and 32–40 h) both amino acid and lipid class metabolites were equally perturbed, commensurate with the stage-dependent development of parasite metabolism. The pathway labelled “Cofactors” corresponds to metabolites that belong to cofactor and vitamin metabolism. The pathway labelled “Other” includes metabolites that do not belong to any of the major pathways