Sample Processing Flowchart. It is observed that 963 samples out 1724 were negatives by RDT and 761 positives. By NM-PCR was detected inside the negative group 128 positive samples (122 Pf, 1 Pm, 3 Po and 1 Pv/Pf), therefore, they were false negatives. The frequency of false negatives by RDT is 7.4%. In these 128 samples were studied the Pfhrp2 and Pfhrp3 genes. All these data are published in a previous article Berzosa et al.