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Table 2 WHO bio-assays results against susceptible An. gambiae s.s. and resistant An. arabiensis

From: Long-lasting insecticidal nets retain bio-efficacy after 5 years of storage: implications for malaria control programmes

Test system

Test item

Washes

Cone test (N = 80)

WHO tunnel test (N = 100)

Pass/fail WHO efficacy criteria (2013)

%KD60 [95% CI]

%24-h mortality [95% CI]

%Feeding inhibition [95% CI]

%24-h mortality [95% CI]

Susceptible

Anopheles gambiae s.s (Ifakara strain)

Olyset®

0

100

03.75 [01.47–06.03]

Pass

1

100

31.64 [29.40–33.89]

Pass

3

96.25 [95.06–97.44]

01.25 [00.06–02.44]

Pass

5

93.75 [89.24–98.26]

0

Pass

10

93.75 [92.56–94.94]

02.50 [01.12–03.88]

Pass

15

83.75 [79.68–87.82]

01.25 [00.06–02.44]

100

90.91

Pass

20

75.00 [69.85–80.15]

03.75 [01.47–06.03]

96.00

51.52

Pass

DawaPlus® 2.0

0

100

92.50 [90.12–94.88]

Pass

1

100

100

Pass

3

100

98.75 [97.56–99.94]

Pass

5

100

97.50 [96.12–98.88]

Pass

10

100

96.25 [92.68–99.80]

Pass

15

100

93.75 [87.79–99.71]

Pass

20

100

100

Pass

Resistant

Anopheles arabiensis (Kingani strain)

Olyset®

0

38.75 [34.68–42.82]

0

1

25.00 [19.85–30.15]

01.25 [00.06–2.44]

3

23.75 [19.68–27.82]

03.75 [01.47–6.03]

96.00

09.18

Pass

5

51.25 [47.18–55.32]

03.75 [02.56–4.94]

87.00

12.24

Fail

10

36.25 [33.25–39.25]

01.25 [00.06–2.44]

87.00

14.29

Fail

15

53.75 [40.79–66.71]

0

92.00

19.39

Pass

20

58.75 [57.56–59.94]

0

88.00

22.45

Fail

DawaPlus® 2.0

0

50.00 [44.85–55.15]

10.00 [05.64–14.35]

1

63.75 [61.47–66.03]

11.25 [06.74–15.76]

3

67.50 [63.37–71.63]

16.25 [15.06–17.44]

90.00

61.62

Pass

5

91.25 [87.68–94.82]

36.25 [31.34–41.16]

94.00

56.57

Pass

10

100

23.75 [19.68–27.82]

94.00

78.79

Pass

15

97.50 [96.12–98.88]

05.00 [01.63–08.37]

68.00

51.52

Pass

20

93.75 [89.24–98.26]

12.50 [09.42–15.58]

93.00

41.41

Pass

  1. N= number of mosquitoes released on each test