Fig. 4

Immune response in mice immunized with rPoMSP4. a IgG was detected at day 7 post-immunization, and the levels continued to rise throughout the whole immunization period. b Immunized mice sera were diluted from 1:10,000 to 1:2,560,000. Data are presented as the geometric mean OD obtained at different concentrations. Numbers on the X-axis indicate the dilutions tested. Antigen specificity was confirmed using pre-immune serum samples as control. c Antibody avidity of rPoMSP4 was verified through ELISA. Antibody avidity was higher in PowMSP4 (mean 92.3) than in PocMSP4 (mean 80.5). d Cross-reaction of PocMSP4 with sera from mice immunized with rPowMSP4 proteins and cross-reaction of PowMSP4 with sera from mice immunized with rPocMSP4 (PC, positive control). e Splenocytes of immunized BALB/c mice were stimulated with 5 µg/mL of each recombinant protein used in immunization. The cells were stimulated for 72 h. Data were measured at 450 nm and transformed in percentages of PocMSP4 (36%) and PowMSP4 (15.8%)