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Fig. 2 | Malaria Journal

Fig. 2

From: Quantifying late-stage host-seeking behaviour of Anopheles gambiae at the insecticidal net interface using a baited-box bioassay

Fig. 2

Activity of adult female Anopheles gambiae s.s. at untreated netting. The three Baited Box studies are shown separately, with behavioural events as defined in the ethogram in Table 1. Each line represents the responses and complete activity of a single mosquito, with duration of each behavioural event measured on the X-axis (0–28 min). On each chronogram, the initial grey-scale borderless sections represent activity prior to initial landing on the netting. The orange red and green segments with black borders are events that include contact with the test netting. In Study 1 (a), all events from initial net contact to proboscis insertion (Events 4 and 5) were combined into a single event. Throughout the test, mosquitoes were allowed to exit the release point and approach the operator thumb, probe, blood feed and depart from the test net without interference. In Studies 1 (a) and 2 (b), post-feeding resting on the net was limited to 3 min whereas in Study 3 (c), post-feeding resting could continue up to a total test duration of 20 min

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