Fig. 3
Entry into a growth-retarded state is dependent on the point in the cell cycle at which isoleucine is removed. Synchronous rings were transferred from normal medium to isoleucine-depleted medium every 3 h over a 45-h cycle. The relative growth was quantified by flow cytometry after 30 h (for > 24 hpi) or 60 h (for < 24 hpi) in isoleucine lacking medium. A logistic regression of relative growth vs. hours post invasion (hpi) shows the likelihood of completing the cell cycle increases with hpi (p = 5.85 × 10−82, see Additional file 1: Table S1 for regression summary). t50 = the time at which 50% of parasites exhibit growth (vertical broken line)