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Table 2 Enrichment of zygotes during purification using “2 MACS 2 Percoll” method

From: Purification and production of Plasmodium falciparum zygotes from in vitro culture using magnetic column and Percoll density gradient

Parasite/Cell

Before Purification (after 6 h in ookinete media)

After 1st MACS column

After 2nd MACS column

After 1st Percoll column

After 2nd Percoll column

Upper band

Lower band

Upper band

Middle band

Lower band

RBC

6.0x109

5.0x106

0

0

0

0

0

0

Gam

35.3x107

2.2x107

5.8x106

1.7x106

2.1x106

0

7.4x105

2.6x105

Zygote

14.4x107

6.7x107

3.4x107

1.7x107

4.8x106

2.8x106

6.0x106

3.0x105

Mac Gam

2.1x107

4.6x106

1.2x106

2.0x105

6.0x105

0

1.5x105

0

Zygote  %

2.2%

67.9%

82.9%

89.9%

64.0%

100%

87.1%

53.5%

  1. Absolute total numbers of RBCs and different parasite stages before and after each purification step are shown. Enrichment of zygote is indicated by the percentage of zygote in the whole population after each purification step
  2. RBC  red blood cells, Gam gametocytes, Mac Gam macrogametocytes