Skip to main content

Table 3 Differentially expressed proteins between malaria groups using 2D-DIGE analysis

From: Depleted circulatory complement-lysis inhibitor (CLI) in childhood cerebral malaria returns to normal with convalescence

SpotGel analysisaIDProtein identificationbMean intensityclog2 (FC) compared to CC
CCUMSMACMUMSMACM
Fibrinogen subunits
1Crude plasmaP02675Fibrinogen beta chain326,0432,395,644188,428342,8172.88− 0.790.07
23ImmunodepletedP02671Fibrinogen alpha chain111,201202,297235,418193,9440.861.080.80
24ImmunodepletedP02671Fibrinogen alpha chain95,490166,776248,306184,9680.801.380.95
26ImmunodepletedP02679Fibrinogen gamma chain89,777167,685193,504177,5220.901.110.98
Acute phase proteins
2Crude plasmaP01011Alpha-1 antichymotrypsin843738,66053,70646,5322.202.672.46
3Crude plasmaP01011Alpha-1 antichymotrypsin25,63747,77454,94742,4690.901.100.73
8Crude plasmaP02764Alpha-1-acid glycoprotein 1194,326326,673691,483742,1100.751.831.93
9Crude plasmaP02764
P19652
Alpha-1-acid glycoprotein 1/248,800193,249327,999515,0031.992.753.40
21ImmunodepletedP01011Alpha-1 antichymotrypsin563,1201,142,7982,002,4471,967,4301.021.831.80
31ImmunodepletedP02750Leucine-rich alpha-2 glycoprotein79,647217,512231,940200,7761.451.541.33
Cholesterol transport
29ImmunodepletedP06727Apolipoprotein AIV107,49154,999395,132249,844− 0.971.881.22
30ImmunodepletedP06727Apolipoprotein AIV31,61119,92945,626115,303− 0.670.531.87
Heme-induced oxidative stress
4Crude plasmaP00738Haptoglobin beta chain121,132102,98121,37488,732− 0.23− 2.50− 0.45
5Crude plasmaP00738Haptoglobin beta chain122,286176,6320114,0070.53 − 0.10
6Crude plasmaP00738Haptoglobin beta chain136,742192,62035,403147,9500.49− 1.950.11
7Crude plasmaP00738Haptoglobin beta chain89,871158,85229,216126,0230.82− 1.620.49
10Crude plasmaP00738Haptoglobin alpha-2-chain51,14878,021025,5450.61 − 1.00
11Crude plasmaP00738Haptoglobin alpha-2-chain20,54578,021028901.93 − 2.83
15Crude plasmaP68871Haemoglobin subunit-beta47,55526,914168,21215,023− 0.821.82− 1.66
16Crude plasmaP69905Haemoglobin subunit-alpha32,20342,761278,353102,6650.413.111.67
17ImmunodepletedP02790Hemopexin1,712,6521,368,897327,700812,479− 0.32− 2.39− 1.08
18ImmunodepletedP02790Hemopexin57,18141,61526,79324,527− 0.46− 1.09− 1.22
19ImmunodepletedP02790Hemopexin601,982411,670171,318345,268− 0.55− 1.81− 0.80
20ImmunodepletedP02790Hemopexin1,788,7431,276,004698,022754,038− 0.49− 1.36− 1.25
Complement-induced oxidative stress
32ImmunodepletedP10909Clusterin47,05531,17618,48030,678− 0.59− 1.35− 0.62
  1. ID Protein Accession Number
  2. aSpot detected as > 1.5-fold significant change compared to other groups identified and analysed from either crude plasma sample or an immunodepleted plasma sample. Representative images and positions of numbered spots are shown in Fig. 1
  3. bSpot identifications are detailed in Additional file 1: Fig. S1
  4. cMean intensity as estimated by Bio-Rad PDQuest software. (n = 2 for crude plasma, n = 4 for immunodepleted)
  5. dLog2 (fold change) mean intensity of protein in each disease case compared to CC