Depleted circulatory complement-lysis inhibitor (CLI) in childhood cerebral malaria returns to normal with convalescence

Abah, Samuel Eneọjọ; Burté, Florence; Howell, Steven A.; Lagunju, Ikeoluwa; Shokunbi, Wuraola A.; Wahlgren, Mats; Sodeinde, Olugbemiro; Brown, Biobele J.; Holder, Anthony A.; Fernandez-Reyes, Delmiro

doi:10.1186/s12936-020-03241-5

Table 3 Differentially expressed proteins between malaria groups using 2D-DIGE analysis

From: Depleted circulatory complement-lysis inhibitor (CLI) in childhood cerebral malaria returns to normal with convalescence

Spot	Gel analysis^a	ID	Protein identification^b	Mean intensity^c				log₂ (FC) compared to CC
Spot	Gel analysis^a	ID	Protein identification^b	CC	UM	SMA	CM	UM	SMA	CM
Fibrinogen subunits
1	Crude plasma	P02675	Fibrinogen beta chain	326,043	2,395,644	188,428	342,817	2.88	− 0.79	0.07
23	Immunodepleted	P02671	Fibrinogen alpha chain	111,201	202,297	235,418	193,944	0.86	1.08	0.80
24	Immunodepleted	P02671	Fibrinogen alpha chain	95,490	166,776	248,306	184,968	0.80	1.38	0.95
26	Immunodepleted	P02679	Fibrinogen gamma chain	89,777	167,685	193,504	177,522	0.90	1.11	0.98
Acute phase proteins
2	Crude plasma	P01011	Alpha-1 antichymotrypsin	8437	38,660	53,706	46,532	2.20	2.67	2.46
3	Crude plasma	P01011	Alpha-1 antichymotrypsin	25,637	47,774	54,947	42,469	0.90	1.10	0.73
8	Crude plasma	P02764	Alpha-1-acid glycoprotein 1	194,326	326,673	691,483	742,110	0.75	1.83	1.93
9	Crude plasma	P02764 P19652	Alpha-1-acid glycoprotein 1/2	48,800	193,249	327,999	515,003	1.99	2.75	3.40
21	Immunodepleted	P01011	Alpha-1 antichymotrypsin	563,120	1,142,798	2,002,447	1,967,430	1.02	1.83	1.80
31	Immunodepleted	P02750	Leucine-rich alpha-2 glycoprotein	79,647	217,512	231,940	200,776	1.45	1.54	1.33
Cholesterol transport
29	Immunodepleted	P06727	Apolipoprotein AIV	107,491	54,999	395,132	249,844	− 0.97	1.88	1.22
30	Immunodepleted	P06727	Apolipoprotein AIV	31,611	19,929	45,626	115,303	− 0.67	0.53	1.87
Heme-induced oxidative stress
4	Crude plasma	P00738	Haptoglobin beta chain	121,132	102,981	21,374	88,732	− 0.23	− 2.50	− 0.45
5	Crude plasma	P00738	Haptoglobin beta chain	122,286	176,632	0	114,007	0.53		− 0.10
6	Crude plasma	P00738	Haptoglobin beta chain	136,742	192,620	35,403	147,950	0.49	− 1.95	0.11
7	Crude plasma	P00738	Haptoglobin beta chain	89,871	158,852	29,216	126,023	0.82	− 1.62	0.49
10	Crude plasma	P00738	Haptoglobin alpha-2-chain	51,148	78,021	0	25,545	0.61		− 1.00
11	Crude plasma	P00738	Haptoglobin alpha-2-chain	20,545	78,021	0	2890	1.93		− 2.83
15	Crude plasma	P68871	Haemoglobin subunit-beta	47,555	26,914	168,212	15,023	− 0.82	1.82	− 1.66
16	Crude plasma	P69905	Haemoglobin subunit-alpha	32,203	42,761	278,353	102,665	0.41	3.11	1.67
17	Immunodepleted	P02790	Hemopexin	1,712,652	1,368,897	327,700	812,479	− 0.32	− 2.39	− 1.08
18	Immunodepleted	P02790	Hemopexin	57,181	41,615	26,793	24,527	− 0.46	− 1.09	− 1.22
19	Immunodepleted	P02790	Hemopexin	601,982	411,670	171,318	345,268	− 0.55	− 1.81	− 0.80
20	Immunodepleted	P02790	Hemopexin	1,788,743	1,276,004	698,022	754,038	− 0.49	− 1.36	− 1.25
Complement-induced oxidative stress
32	Immunodepleted	P10909	Clusterin	47,055	31,176	18,480	30,678	− 0.59	− 1.35	− 0.62

ID Protein Accession Number
^aSpot detected as > 1.5-fold significant change compared to other groups identified and analysed from either crude plasma sample or an immunodepleted plasma sample. Representative images and positions of numbered spots are shown in Fig. 1
^bSpot identifications are detailed in Additional file 1: Fig. S1
^cMean intensity as estimated by Bio-Rad PDQuest software. (n = 2 for crude plasma, n = 4 for immunodepleted)
^dLog₂ (fold change) mean intensity of protein in each disease case compared to CC

Back to article page

ISSN: 1475-2875

Contact us

Submission enquiries: ayesha.siddiqka@springernature.com
General enquiries: info@biomedcentral.com

Malaria Journal

Contact us