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Fig. 2 | Malaria Journal

Fig. 2

From: Measuring antibody avidity to Plasmodium falciparum merozoite antigens using a multiplex immunoassay approach

Fig. 2

Avidity indexes after treatment with 8 M urea and 1 M, 2 M and 3 M NH4SCN. The same PC and samples from 5 individual adults used in Fig. 1 were re-tested in this experiment. Following incubation of plasma with the antigen-coupled beads, the complexes were incubated with 8 M urea or 1 M, 2 M and 3 M or NH4SCN for 30 min and amount of Ab remaining bound (AI) were calculated. Error bars represent the mean ± one SD for 3 replicates. Baseline MFI values [mean ± SD based on 15 replicates in the absence of salt] were as follows: a AMA-1 23,958 ± 98 MFI; b EBA-175: 22,413 ± 166; c MSP1 3D7: 10,169 ± 208 MFI; d MSP3: 2122 ± 82MFI. OPTIONAL INSERT: Comparison of data in Figs. 1 and 2 show that the majority of mean AI have overlapping SD; however, a few differences are seen, especially with 8 M urea for AMA1 and MSP3 that are due to inter-assay variation

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