Fig. 3

Comparison of the length of incubation with the chaotrope. Antigen-coupled beads were incubated with the pooled Positive Control (PC) for 60 min. Beads were then washed, incubated with 4 M GdHCl, 8 M urea, or 1 M, 2 M or 3 M NH₄SCN for either 15 or 30 min at RT on a rotary shaker. Following washing and incubation with R-phycoerythrin-labeled, Goat anti-human IgG (Fc fragment) for 60 min, MFI were recorded and AI calculated. Error bars represent mean ± SD based on 3 replicates. Baseline MFI values [mean ± SD MFI for 30 replicates in the absence of salt] were as follows: AMA-1 23,955 ± 133 MFI; EBA-175 22,473 ± 587; MSP1 (3D7) 10,042 ± 333 MFI; and MSP3: 1990 ± 155 MFI