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Table 1 Validation of TaqMan assays with laboratory isolates

From: Development of copy number assays for detection and surveillance of piperaquine resistance associated plasmepsin 2/3 copy number variation in Plasmodium falciparum

StrainAssayReplicatesPlasmepsin Est. FCMDR-1 Est. FC
AvgSD (range)AvgSD (range)
3D7plasmepsin 281.000.02 (0.97–1.04)
Dd2plasmepsin 281.060.03 (1.03–1.12)
PH1097-Cplasmepsin 261.060.02 (1.04–1.09)
PH1265-Cplasmepsin 261.890.02 (1.85–1.93)
PH1310-Cplasmepsin 261.060.03 (1.01–1.13)
PH1387-Cplasmepsin 261.930.09 (1.8–2.04)
3D7plasmepsin 381.020.03 (0.96–1.08)
Dd2plasmepsin 381.090.03 (1.05–1.15)
PH1097-Cplasmepsin 361.060.02 (1.03–1.09)
PH1265-Cplasmepsin 362.050.06 (1.93–2.14)
PH1310-Cplasmepsin 361.000.03 (1.02–1.09)
PH1387-Cplasmepsin 362.290.07 (2.19–2.38)
3D7plasmepsin 2/pfmdr181.010.02 (0.97–1.04)1.000.02 (0.96–1.02)
Dd2plasmepsin 2/pfmdr181.100.04 (1.04–1.17)1.830.06 (1.77–1.92)
PH1097-Cplasmepsin 2/pfmdr161.070.05 (1–1.13)1.060.05 (1.01–1.11)
PH1265-Cplasmepsin 2/pfmdr161.800.03 (1.75–1.84)0.960.02 (0.93–0.99)
PH1310-Cplasmepsin 2/pfmdr161.020.02 (1–1.06)1.010.02 (0.98–1.05)
PH1387-Cplasmepsin 2/pfmdr161.890.05 (1.84–2)0.930.03 (0.91–0.99)