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Table 1 Validation of TaqMan assays with laboratory isolates

From: Development of copy number assays for detection and surveillance of piperaquine resistance associated plasmepsin 2/3 copy number variation in Plasmodium falciparum

Strain

Assay

Replicates

Plasmepsin Est. FC

MDR-1 Est. FC

Avg

SD (range)

Avg

SD (range)

3D7

plasmepsin 2

8

1.00

0.02 (0.97–1.04)

–

–

Dd2

plasmepsin 2

8

1.06

0.03 (1.03–1.12)

–

–

PH1097-C

plasmepsin 2

6

1.06

0.02 (1.04–1.09)

–

–

PH1265-C

plasmepsin 2

6

1.89

0.02 (1.85–1.93)

–

–

PH1310-C

plasmepsin 2

6

1.06

0.03 (1.01–1.13)

–

–

PH1387-C

plasmepsin 2

6

1.93

0.09 (1.8–2.04)

–

–

3D7

plasmepsin 3

8

1.02

0.03 (0.96–1.08)

–

–

Dd2

plasmepsin 3

8

1.09

0.03 (1.05–1.15)

–

–

PH1097-C

plasmepsin 3

6

1.06

0.02 (1.03–1.09)

–

–

PH1265-C

plasmepsin 3

6

2.05

0.06 (1.93–2.14)

–

–

PH1310-C

plasmepsin 3

6

1.00

0.03 (1.02–1.09)

–

–

PH1387-C

plasmepsin 3

6

2.29

0.07 (2.19–2.38)

–

–

3D7

plasmepsin 2/pfmdr1

8

1.01

0.02 (0.97–1.04)

1.00

0.02 (0.96–1.02)

Dd2

plasmepsin 2/pfmdr1

8

1.10

0.04 (1.04–1.17)

1.83

0.06 (1.77–1.92)

PH1097-C

plasmepsin 2/pfmdr1

6

1.07

0.05 (1–1.13)

1.06

0.05 (1.01–1.11)

PH1265-C

plasmepsin 2/pfmdr1

6

1.80

0.03 (1.75–1.84)

0.96

0.02 (0.93–0.99)

PH1310-C

plasmepsin 2/pfmdr1

6

1.02

0.02 (1–1.06)

1.01

0.02 (0.98–1.05)

PH1387-C

plasmepsin 2/pfmdr1

6

1.89

0.05 (1.84–2)

0.93

0.03 (0.91–0.99)