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Table 1 Details of Primers used in genetic evaluation of Pfdhfr and Pfdhps SNPs in Plasmodium falciparum isolates from Msambweni County Referral Hospital, Kwale, Kenya

From: Prevalence of pfdhfr and pfdhps mutations in Plasmodium falciparum associated with drug resistance among pregnant women receiving IPTp-SP at Msambweni County Referral Hospital, Kwale County, Kenya

No.

Primer name

5′ end

Sequence

3′ end

Amount

Modified Oligo

1

P.falc F

GCTCTTTCTTGATTTCTTGGATG

0.3 uM

No

2

P.falc R

AGCAGGTTAAGATCTCGTTCG

0.3 uM

No

3

P.falc P

CACGAACTAAAAACGGCCAT

0.2 uM

FAM-BHQ1

4

DHFR-51 F

TGAGGTTTTTAATAACTACACATTTAGAGGTCT

0.3 uM

No

5

DHFR-51 R

TATCATTTACATTATCCACAGTTTCTTTGTT

0.3 uM

No

6

DHFR-51 WTP

AATGTAATTCCCTAGATATG

0.2 uM

FAM-BHQ1

7

DHFR-51 MP

AAATGTATTTCCCTAGATATG

0.2 uM

HEX-BHQ1

8

DHFR-59 F

TGAGGTTTTTAATAACTACACATTTAGAGGTCT

0.3 uM

No

9

DHFR-59 R

TATCATTTACATTATCCACAGTTTCTTTGTT

0.3 uM

No

10

DHFR-59 WTP

AATATTTTTGTGCAGTTACA

0.2 uM

FAM-BHQ1

11

DHFR-59 MP

TGAAATATTTTCGTGCAGTTA

0.2 uM

HEX-BHQ1

12

DHFR-108 F

TGGATAATGTAAATGATATGCCTAATTCTAA

0.3 uM

No

13

DHFR-108 R

AATCTTCTTTTTTTAAGGTTCTAGACAATATAACA

0.3 uM

No

14

DHFR-108 WTP

AGAACAAGCTGGGAAA

0.2 uM

FAM-BHQ1

15

DHFR-108 MP

AGAACAAACTGGGAAAG

0.2 uM

HEX-BHQ1

16

DHPS-437 F

TGAAATGATAAATGAAGGTGCTAGTGT

0.9 uM

No

17

DHPS-437 R

AATACAGGTACTACTAAATCTCTTTCACTAATTTTT

0.9 uM

No

18

DHPS-437 WTP

AGAATCCTCTGCTCCT

0.2 uM

FAM-BHQ1

19

DHPS-437 MP

AATCCTCTGGTCCTTT

0.2 uM

HEX-BHQ1

20

DHPS-540 F

AATGCATAAAAGAGGAAATCCACAT

0.3 uM

No

21

DHPS-540 R

TCGCAAATCCTAATCCAATATCAA

0.3 uM

No

22

DHPS-540 WTP

CAATGGATAAACTAACAAA

0.2 uM

FAM-BHQ1

23

DHPS-540 MP

AATGGATGAACTAACAAA

0.2 uM

HEX-BHQ1