Fig. 1

Generation of P. vivax salivary gland sporozoites. a Thin blood smear from a P. vivax infected patient stained with Giemsa, showing the presence of a P. vivax gametocyte. b Mercurochrome stained midguts from mosquitoes fed with P. vivax infected blood, dissected 7 days post infection showing the presence of oocysts. c Salivary gland dissected from P. vivax infected mosquito 14 days post infection. Image shows release of P. vivax sporozoites, as indicated by an arrowhead. Scale bar is 20 μm. d Salivary gland sporozoite dissected from P. vivax infected mosquito immunostained for pvCSP