Fig. 5

Infectivity of iPSC derived hepatocytes from P. vivax infected patients. a Detection of P. vivax EEFs in patient iPSC-derived hepatocytes with P. vivax sporozoites at 4 days post infection. Green signal indicates immuno-staining with P. vivax-specific anti-HSP70 antibody, blue colour indicates DAPI nuclear staining. Scale bar is 50 μm. b Quantitation of number of EEF’s per well for different patient iPSC derived hepatocytes. K1 and H9 are hepatocytes derived from healthy volunteers and ESC, respectively. Error bar represents standard deviation of replicates. Data are pooled from 6 wells per condition, and are representative of three independent infections