Table 2 pfs25 (female gametocyte) qRT-PCR assay for assessing intra-assay variability between technical replicates

5.71 × 10⁶

36/36 (100.0%)

17.14 (0.19)

16.67–17.62

1.1

5.71 × 10⁵

34/34 (100.0%)

20.53 (0.23)

19.87–20.96

1.1

5.71 × 10⁴

35/35 (100.0%)

23.94 (0.18)

23.60–24.33

0.7

5.71 × 10³

36/36 (100.0%)

27.13 (0.19)

26.57–27.49

0.7

5.71 × 10²

36/36 (100.0%)

30.57 (0.22)

30.09–31.29

0.7

5.71 × 10¹

36/36 (100.0%)

33.87 (0.25)

33.10–34.50

0.8

1.15 × 10¹

21/21 (100.0%)

36.15 (0.45)

35.36–36.94

1.2

7.16

21/21 (100.0%)

37.26 (0.55)

36.40–38.87

1.5

5.71

14/15 (93.3%)

38.09 (0.92)

36.81–39.65

2.4

2.86

18/21 (85.7%)

38.58 (1.09)

37.23–40.96

2.8

1.43

16/21 (76.2%)

39.77 (1.01)

38.43–42.32

2.5

0.72

32/42 (76.2%)

40.07 (0.80)

38.92–41.8

2.0

0.36

8/21 (38.1%)

40.42 (0.80)

38.99–42.17

2.0

0.18

3/20 (15.0%)

40.51 (n/a^a)

40.43–40.56

n/a

Negative control

0/33

Not detected

Total

346/395 (87.6%)

33.14 (0.52^b)

16.67–42.32