Table 3 pfMGET (male gametocyte) qRT-PCR assay for assessing intra-assay variability between technical replicates
Standard (gametocytes/mL) | Detected (% positive) | Mean Cq (SD) | Range Cq | %CV Cq |
|---|---|---|---|---|
1.73 × 107 | 15/15 (100.0%) | 16.56 (0.06) | 16.44–16.70 | 0.4 |
1.73 × 106 | 15/15 (100.0%) | 19.86 (0.06) | 19.70–20.08 | 0.3 |
1.73 × 105 | 15/15 (100.0%) | 23.19 (0.06) | 23.03–23.32 | 0.3 |
1.73 × 104 | 15/15 (100.0%) | 26.57 (0.10) | 26.41–26.76 | 0.4 |
1.73 × 103 | 15/15 (100.0%) | 29.94 (0.20) | 29.68–30.52 | 0.7 |
1.73 × 102 | 14/14 (100.0%) | 33.28 (0.39) | 32.63–33.93 | 1.2 |
86.19 | 24/24 (100.0%) | 34.35 (0.40) | 33.32–35.08 | 1.2 |
43.10 | 24/24 (100.0%) | 35.47 (0.92) | 34.09–37.13 | 2.6 |
21.55 | 22/24 (91.7%) | 36.47 (0.75) | 34.94–37.25 | 2.1 |
11.17 | 14/24 (58.3%) | 36.59 (0.49) | 35.47–37.21 | 1.3 |
5.59 | 8/24 (33.3%) | 36.73 (0.64) | 35.89–37.47 | 1.7 |
Negative control | 0/6 | Not detected | Â | Â |
Total | 181/209 (86.6%) | 30.34 (0.51b) | 16.44–37.47 |  |