Fig. 5

Effect of CoPPIX on chemokines release and expression of VCAM-1. Animals received daily i.p. injection of saline with 0.5% DMSO (Saline) or 5 mg/kg CoPPIX (CoPPIX) and were euthanized 9 days after infection. The lungs, BALF and peripheral blood were collected and processed for ELISA. A. CCL-2/MCP-1, and CCL-5/RANTES in lungs (left panels), BAL (middle panels) and plasma (right panels). Each is the mean from 5 animals. The experiment was repeated three times with similar results. *p ≤ 0.05 when compared to uninfected controls. b Lung samples from control and infected animals treated or not with CoPPIX were collected and used for analysis for VCAM-1 expression by western blot. β-bactin was used as normalizer (upper panel). Densitometry analysis is shown in the bottom panel. This is representative of five animals. (#) indicates p ≤ 0.05 when compared to CoPPIX treated animals. P values were calculated using an unpaired Ordinary one-way ANOVA test