Fig. 6

CoPPIX treatment decreases platelet activation in P. berghei NK65 infected animals. C57B1/6 animals were inoculated i.p. with 10⁴ non-parasitized red blood cells (NP-RBC) or with 10⁴ parasitized red blood cells (PRBC). Animals received daily i.p. injection of saline with 0.5% DMSO (Saline) or 5 mg/kg CoPPIX (CoPPIX). Blood was collected from the tail vein at various times throughout the experiment. A. Platelet counts over a 7-day period. At day 7, cardiac puncture was performed and the blood was processed for cytometry. B. Analysis of the formation of platelet/monocytes heteroaggregates, by labelling platelets with CD41a⁺ and monocytes with CD14. C. Analysis of the formation of platelet/neutrophils heteroaggregates by labelling platelets with CD41a⁺ and neutrophils with Ly6-Gr1.CD11b⁺. D. BALF was collected processed for PF4 detection by ELISA. Each bar is the mean from 5 animals. *p ≤ 0.05 when compared to uninfected controls, and (#) indicates p ≤ 0.05 when compared to CoPPIX treated animals. P values were calculated using an unpaired Ordinary one-way ANOVA test